While we are familiar with the Propionate ester the remaining three esters that create Sustanon-250 are almost always found as part of a mixture or compounded anabolic androgenic steroid .

Developed by Organon, the original idea behind Sustanon-250 was to provide a testosterone form well-suited for hormone replacement therapy that would only needed to be administered once every few weeks and for all intense purposes the idea was a success. For the performance enhancing athlete Sustanon-250 can be a fine choice but the idea of injecting only once or twice a month is not applicable here. As a performance enhancer this testosterone like all forms will need to be administered on a more frequent basis. This mixture carries with it two fast, short esters, Propionate and Pheylpropionate, a longer more moderate ester Isocaproate and the very slow and long Decanoate ester. In order to keep testosterone levels stable and at their peak most athletes will inject Sustanon-250 at a minimum of every 3 days and more commonly every other day for optimal results.

For more info see: Sustanon-250

Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more [38] or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram. [39] [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography . [2] : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. [39]




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